PDCD6 encodes a calcium-binding protein belonging to the penta-EF-hand protein family.
Calcium binding is important for homodimerization and for conformational changes required for binding to other protein partners.
This gene product participates in T cell receptor-, Fas-, and glucocorticoid-induced programmed cell death.
In mice deficient for this gene product, however, apoptosis was not blocked suggesting this gene product is functionally redundant.
ALG2 is expressed as a 22kD protein before FAS activation and thereafter as a 19kD protein, probably due to N-terminal cleavage.
Confocal microscopy and immunoprecipitation analysis demonstrated that ALG2 translocates from the cytoplasmic membrane to the cytosol during FAS-induced apoptosis and then dissociates from FAS after activation.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human PDCD6.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Phe32-Val191 of human PDCD6, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
