(2001) showed that the IgV region of mouse Pdl2, like Pdl1, binds to human PDCD1 but not to CTLA4, CD28, or ICOS.
Stimulation of mouse T cells with anti-CD3 and Pdl2 or Pdl1 resulted in inhibition of proliferation and cytokine production, and Pdl2 could inhibit T-cell receptor -CD28 signals at low antigen concentrations.
Latchman et al.
(2001) proposed that blocking of the PDL-PDCD1 pathway may enhance antitumor immunity, whereas stimulating this pathway may help downregulate graft rejection and autoimmune and allergic reactions
Latchman et al.
(2001) mapped the PDL2 gene to chromosome 9p24.2, in close proximity to the PDL1 gene.
Tseng et al.
(2001) mapped the mouse Pdl2 gene to chromosome 19C2-C3.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human PDCD1LG2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Glu33-Phe236 of human PDCD1LG2, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
