Prenylcysteine is released during the degradation of prenylated proteins.
PCYOX1 catalyzes the degradation of prenylcysteine to yield free cysteines and a hydrophobic isoprenoid product
The deduced 505aa protein contains a predicted signal sequence, several transmembrane regions, and potential N-glycosylation sites.
Northern blot analysis detected a 6-kb transcript in heart, kidney, liver, colon, placenta, skeletal muscle, and pancreas, with low expression in brain and no expression in spleen.
The 3-prime untranslated region contains several SINEs, including Alu and MIR sequences.
The predicted protein has 506aa.
RT-PCR ELISA detected high to moderate expression in all adult and fetal human tissues and all specific brain regions tested.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse PCYOX1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Ala249-Leu505 of mouse PCYOX1, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
