These precursors contain amino- and carboxyl-terminal peptide extensions known as N- and C-propeptides, respectively, which are cleaved, upon secretion of procollagen from the cell, to yield the mature triple helical, highly structured fibrils.
In the mouse, procollagen C-proteinase depends for full expression upon the presence of either a 55kD glycoprotein or smaller 36- and 34kD proteolytically processed forms of the same protein.
None of the 3 enhancer proteins exhibits intrinsic procollagen processing activity, but all are capable of enhancing the activity of the C-proteinase by approximately one order of magnitude.
type I procollagen COOH-terminal proteinase enhancer, a glycoprotein that binds to the COOH-terminal propeptide of type I procollagen and enhances C-proteinase activity, from mouse fibroblast culture medium.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
