Protein C inhibitor (PCI, SERPINA5) is a serine protease inhibitor (serpin) which limits the expression of protein C.Suzuki et al.
(1987) determined the sequence of cDNA for human protein C inhibitor (PCI).
A high degree of homology of the deduced amino acid sequence to that of other known inhibitors clearly demonstrated that protein C inhibitor is a member of the superfamily of serine protease inhibitors including alpha-1-antichymotrypsin (AACT), alpha-1-antitrypsin (AAT; PI), antithrombin III (AT3), and angiotensinogen (AGT).
Protein C inhibitor is sometimes referred to as plasminogen activator inhibitor-3 (PAI3) because it also inhibits plasminogen activators (Meijers and Chung, 1991).
Plasminogen activator inhibitor-1 (PAI1) has structural similarities to PCI.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human PCI.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Ser43-Pro406 of human PCI, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
