Cadherins are a group of integral membrane proteins that mediate calcium-dependent cell-cell adhesion.
By PCR of rat, mouse, and human neural cell cDNA with degenerate primers based on conserved regions of the cadherin extracellular domain, Sano et al.
(1993) isolated partial cDNAs encoding pc42 and pc43 (PCDH2).
They used the partial cDNAs to screen a human brain library and recovered cDNAs corresponding to the entire coding sequence of both genes
Like typical cadherins, both pc42 and pc43 contain an N-terminal extracellular domain, a transmembrane domain, and a C-terminal cytoplasmic region.
The extracellular domains of pc42 and pc43 contain 7 and 6 repeats, respectively, of a subdomain that is very similar to those of cadherins, but with characteristic properties.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse PCDH1. Used in Western Blot, Sample: Recombinant PCDH1, Mouse
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Val246-Ala517 of mouse PCDH1, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
