84-4431-35　［受注停止］PARN (Poly A Specific Ribonuclease, DAN, Deadenylation Nuclease, Deadenylating nuclease, Polyadenylate-specific ribonuclease) 200ul　364525

特徴

• Poly A Specific Ribonuclease is a 3'-exoribonuclease, with similarity to the RNase D family of 3'-exonucleases.
• It prefers poly(A) as the substrate, hence, efficiently degrades poly(A) tails of mRNAs.
• Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs.
• This protein is also involved in silencing of certain maternal mRNAs during oocyte maturation and early embryonic development, as well as in nonsense-mediated decay (NMD) of mRNAs that contain premature stop codons.
• Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
• Poly(A)-specific ribonuclease PARN protein appears to be localized in both the nucleus and the cytoplasm.
• It is not stably associated with polysomes or ribosomal subunits.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.