Protease-activated receptors 1 (PAR1), 2 (PAR2), and 3 (PAR3) are members of a unique G protein-coupled receptor family.
They are characterized by a tethered peptide ligand at the extracellular amino terminus that is generated by minor proteolysis.
By fluorescence in situ hybridization, Xu et al.
(1998) mapped the PAR4 gene to 19p12
Coagulation factor II (thrombin) receptor-like 3 (F2RL3) is a member of the large family of 7-transmembrane-region receptors that couple to guanosine-nucleotide-binding proteins.
F2RL3 is also a member of the protease-activated receptor family.
F2RL3 is activated by proteolytic cleavage of its extracellular amino terminus.
The new amino terminus functions as a tethered ligand and activates the receptor.
F2RL3 is activated by thrombin and trypsin.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human PAR4.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Gly48-Gln385 of human PAR4, fused to two N-terminal Tags, His-Tag and GST-tag, expressed in E. coli.
