Pappalysins comprise one of the five families of Metzincins (other families include ADAMs/ADAMTSs, MMPs, Astacins, and Serralysins).
Pappalysin-1 (PAPP-A) is an important pregnancy protein and increases in plasma by a factor of about 150 during pregnancy.
Reduced maternal serum PAPP-A is a major marker of Down syndrome in the first trimester of pregnancy.
PAPP-A cleaves IGFBP-4 and -5 (insulin-like growth factor-binding protein-4 and 5) at a single site, resulting in the release of bioactive IGF
Pappalysin-2 (PAPP-A2; also called PAPP-E) shares 45%aa identity with PAPP-A and cleaves IGFBP-2.sialic acid binding Ig-like lectin 6 (Siglec-6), a potential leptin receptor, and pappalysin-2 (PAPP-A2), a protease that cleaves IGF-binding proteins
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human PAPPA2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to His1309-Ser1492 of human PAPPA2, fused to N-terminal His-Tag expressed in E. coli.
