PAPP-A is a large heterottetrameric glycoprotein of ~500kD, which was first discovered in serum from pregnant women.
The hetreotetramer consists of two PAPP-A subunits and two proMBP subunits.
Low maternal serum levels of PAPP-A in first trimester biochemical screening is used as a marker of Down’s syndrome.
a secreted metalloproteinase which cleaves insulin-like growth factor binding proteins (IGFBPs).
It is thought to be involved in local proliferative processes such as wound healing and bone remodeling.
Low plasma level of this protein has been suggested as a biochemical marker for pregnancies with aneuploid fetuses (fetuses with an abnormal number of chromosomes).
For example, low PAPPA may be seen in prenatal screening for Down syndrome.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human PAPPA.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Ala244-Leu501of human PAPPA, fused to N-terminal His-Tag expressed in E. coli.
