PAK2 shares 73%aa identity with rat brain Pak65, including more than 95% identity within the kinase domain; PAK2 also shares homology with the yeast Ste20 gene.
PAK2 is ubiquitously expressed, with highest levels detected in skeletal muscle, ovary, thymus, and spleen.
PAK2 transcripts of 7.5, 5, 4.4, and 3 kb were detected in most tissues.
The 7.5-kb transcript was predominant in all tissues tested except muscle, where the 7.5- and 3-kb transcripts were equally expressed.
In brain, a 3.3-kb transcript was expressed.
the multiple PAK2 mRNAs result from alternative splicing.
Western blot analysis using an antibody against rat brain Pak65 detected a brain-specific PAK protein, whereas a human PAK2 antibody detected PAK proteins in heart, kidney, liver, and very strongly in purified neutrophil PAK2.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human PAK2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Glu177-Ala419 of human PAK2, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
