PAG1 is a type III transmembrane adaptor protein that binds to the tyrosine kinase csk protein.
It is thought to be involved in the regulation of T cell activation.Initiation of signal transduction via immunoreceptors depends on interactions of the engaged receptor with glycosphingolipid-enriched membrane microdomains (GEMs), also known as membrane rafts.
GEMs are small signaling islets that are relatively resistant to detergent solubilization except by n-alkyl glycosides.
Sequence analysis predicted that the 432aa type III transmembrane protein contains a 16aa extracellular domain, a 20aa transmembrane domain, a putative palmitoylation site, and a 397aa cytoplasmic tail with 9 potential tyrosine phosphorylation sites and multiple putative casein kinase II and protein kinase C phosphorylation sites.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat PAG!.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Ile138-Phe357 of rat PAG1, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
