84-4431-20　PAG1 (Phosphoprotein Associated With Glycosphingolipid Microdomains 1, CBP, PAG, Csk-Binding Protein, Transmembrane adapter protein PAG, Transmembrane phosphoprotein Cbp) 200ul　364510

特徴

• PAG1 is a type III transmembrane adaptor protein that binds to the tyrosine kinase csk protein.
• It is thought to be involved in the regulation of T cell activation.Initiation of signal transduction via immunoreceptors depends on interactions of the engaged receptor with glycosphingolipid-enriched membrane microdomains (GEMs), also known as membrane rafts.
• GEMs are small signaling islets that are relatively resistant to detergent solubilization except by n-alkyl glycosides.
• Sequence analysis predicted that the 432aa type III transmembrane protein contains a 16aa extracellular domain, a 20aa transmembrane domain, a putative palmitoylation site, and a 397aa cytoplasmic tail with 9 potential tyrosine phosphorylation sites and multiple putative casein kinase II and protein kinase C phosphorylation sites.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
• AA Sequence:MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- G VEGPYEVLKD SSSQENMVED CLYETVKEIK EVADKGQGGK SKSTSALKEL QGAPMEGKAD FAEYASVDRN KKCRHSANAE SILGTCSDLD EESPPPVPVK LLDENANLPQ EGGGQAEEQA AEGTGGHSKR FSSLSYKSRE EDPTLTEEEI SAMYSSVNKP GQSAHKPGPC MKGPESACHS MKGLPQRSSS SCNDLYATVK DFEKTPNSIS TLPPARRPSE EPEPDYEAIQ
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.