Platelet-activating factor acetylhydrolase 2, cytoplasmic is a single-subunit intracellular enzyme that catalyzes the removal of the acetyl group at the SN-2 position of platelet-activating factor (identified as 1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine).
However, this lipase exhibits a broader substrate specificity than simply platelet activating factor.
Two other isoforms of intracellular platelet-activating factor acetylhydrolase exist, and both are multi-subunit enzymes
Additionally, there is a single-subunit serum isoform of this enzyme.
PAFAH2 shares approximately 43% identity with plasma PAFAH, but has virtually no similarity to brain PAFAH1B1.
Unlike PAFAH, PAFAH2 contains no secretory signal sequence, confirming its intracellular localization.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human PAFAH2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met1-Leu392 of human PAFAH2, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
