84-4431-18　PADI6 (Peptidyl Arginine Deiminase Type VI, Peptidylarginine deiminase VI, Peptidyl arginine deiminase-like protein) 200ul　364508

特徴

• Peptidylarginine deiminases (PADs) convert arginine residues in proteins into citrullines.
• Peptidylarginine deiminases (PADs, EC 3.5.3.15), including PADI6, make up a family of posttranslational protein modification enzymes that convert arginine residues to citrulline residues in the presence of calcium ions
• PADI6,The deduced 694aa protein has a calculated molecular mass of 77.7kD.
• RT-PCR detected expression in adult human tissues, including ovary, peripheral blood leukocytes, and testis, with weaker expression in small intestine, spleen, lung, liver, and skeletal muscle.
• No expression was detected in other tissues examined.
• The order of the genes is p-telomere--PADI2--PADI1--PADI3--PADI4--PADI6.
• Mouse Padi6 maps to a syntenic Pad cluster on chromosome 4E1.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry.
• Other applications not tested.
• Recommended Dilutions:ELISA: 0.05-2ug/mlWestern Blot: 0.5-2ug/mlImmunohistochemistry (frozen): 5-20ug/mlImmunohistochemistry (Paraffin): 5-20ug/ml Immunocytochemistry: 5-20ug/mlOptimal dilutions to be determined by the researcher.
• Positive Control Included:364508A: PADI6, Positive ControlSuppled as a recombinant protein in loading buffer (100mM Tris, pH 6.8, 1% SDS, 150mM sodium chloride, 50mM DTT, 0.02% BPB, 0.02% sodium azide, 50% glycerol)
• DAB: 10ul/wellECL: 5ul/wellStore at -20°C.
• Stable for 6 months after receipt.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.