PADI4 is a member of a gene family which encodes enzymes responsible for the conversion of arginine residues to citrulline residues.
This gene may play a role in granulocyte and macrophage development leading to inflammation and immune response
The deduced 663aa protein, approximately 50% identical to rat PAD types I, II, and III and 73% identical with rat keratinocyte PAD IV, is most conserved in the C terminal two-thirds of the sequence.
Functional analysis indicated that the recombinant protein is fully active against arginine-containing substrates.
Western blot analysis showed that expression of a 67kD protein is induced by incubation of HL60 cells with differentiation factors.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human PADI4.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met1-Pro300 of human PADI4, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
