OXCT1encodes a member of the 3-oxoacid CoA-transferase gene family.
The encoded protein is a homodimeric mitochondrial matrix enzyme that plays a central role in extrahepatic ketone body catabolism by catalyzing the reversible transfer of coenzyme A from succinyl-CoA to acetoacetate.
Mutations in this gene are associated with succinyl CoA:3-oxoacid CoA transferase deficiency.OXCT1 is a mitochondrial matrix enzyme that functions as a homodimer.
From human heart cDNA libraries, Kassovska-Bratinova et al.
(1996) cloned cDNAs spanning the entire 1,560-bp coding sequence using pig heart OXCT1 cDNA as a probe.
They reported that a single mRNA transcript of 3.2kb was present in human tissues.
OXCT1 mRNA was most abundant in heart and was also detectable in leukocytes and fibroblasts.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human OXCT1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Thr40-Leu489 of human OXCT1, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
