(2002) isolated a cDNA encoding Olfm3, which they termed optimedin, from a rat eye angle cDNA library.
By database analysis, they identified human OLFM3, and by screening a mouse genomic BAC library, they isolated mouse Olfm3.
They identified 2 main Olfm3 splice variants in rodents, optimedin A and optimedin B, which encode proteins of 458 and 478aa, respectively, that differ in their N termini.
Mouse and rat optimedins share 99% aa identity with human optimedin.
Northern blot analysis detected rat optimedin expression in eye angle tissues, retina, and brain, with lower expression in lens.
RT-PCR analysis showed that optimedin A was preferentially expressed in adult rat retina and brain, while optimedin B was expressed in rat eye angle.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human OLFM3.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Gln24-Glu198 of human PLFM3, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
