84-4430-87　OCT4 (Octamer Binding Transcription Factor 4, POU5F1, OCT3, OTF3, OTF4, Octamer-4, POU Domain Class 5 Transcription Factor 1, POU Class 5 Homoeobox 1, Octamer-binding protein 3) 200ul　364478

特徴

• Oct-4 is a homeodomain transcription factor of the POU family.
• This protein is critically involved in the self-renewal of undifferentiated embryonic stem cells.
• As such, it is frequently used as a marker for undifferentiated cells.
• Oct-4 expression must be closely regulated; too much or too little will actually cause differentiation of the cells.
• Oct-4 transcription factor is initially active as a maternal factor in the oocyte but remains active in embryos throughout the preimplantation period.
• Oct-4 expression is associated with an undifferentiated phenotype and tumors.
• In fact gene knockdown of Oct-4 promotes differentiation, thereby demonstrating a role for these factors in human embryonic stem cell self-renewal.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
• AA Sequence:MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- SQ DMKALQKELE QFAKLLKQKR ITLGYTQADV GLTLGVLFGK VFSQTTICRF EALQLSLKNM CKLRPLLEKW VEEADNNENL QEICKSETLV QARKRKRTSI ENRVRWSLET MFLKCPKPSL QQITHIANQL GLEKDVVRVW FCNRRQKGKR SSIEYSQREE YEATGTPFPG GAVSFPLPPG PHFGTPGYGS PHFTTLYSVP FPEGEAFPSV PVTALGSPMH SN
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.