An understanding of development necessitates an understanding of the molecular mechanisms of cell type-specific gene expression.
Using synthetic promoter constructions, it has been found that 2 sequences within immunoglobulin promoters are sufficient for lymphoid-specific promoter activity: an octamer, ATTTGCAT, and a TATA box.
Staudt et al.
(1988) isolated a cDNA which hybridized with mRNA transcripts that were largely restricted to lymphoid cells.
The level of expression of the gene, termed OCT2, within different lymphoid cell lines correlated well with the amount of a nuclear factor called NFA2 that had previously been detected only in lymphoid cells.
Ko et al.
(1988) determined the DNA sequence of the OCT2 cDNA and showed that its gene product contains a homeobox.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat OCT2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Ala49-Ile288 of rat OCT2, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
