84-4430-83　OCT1 (Octamer Binding Transcription Factor 1, POU2F1, OTF1, NF-A1, POU Domain,Class 2,Transcription Factor 1) 200ul　364474

特徴

• POU domain proteins contain a bipartite DNA-binding domain divided by a flexible linker that enables them to adopt various monomer configurations on DNA.
• The versatility of POU protein operation is additionally conferred at the dimerization level.
• Tomilin et al.
• (2000) found that the POU dimer from the OCT1 gene formed on the palindromic OCT factor recognition element, or PORE (ATTTGAAATGCAAAT), could recruit the transcriptional coactivator OBF1, whereas POU dimers formed on the consensus MORE or on MOREs from immunoglobulin heavy chain promoters failed to interact.
• An interaction with OBF1 was precluded since the same OCT1 residues that form the MORE dimerization interface are also used for OBF1/OCT1 interactions on the PORE.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
• AA Sequence:MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGSEF-AGG QITGLTLTPA QQQLLLQQAQ AQAQLLAAAV QQHSASQQHS AAGATISASA ATPMTQIPLS QPIQIAQDLQ QLQQLQQQNL NLQQFVLVHP TTNLQPAQFI ISQTPQGQQG LLQAQNLLTQ LPQQSQANLL QPQPSITLTS QPTTPTRTIA AASVQTLPQS QSTPKRIDTP SLEEPSDLEE LEQFAKTFKQ RRIKLGFTQG DVGLAMGKLY GNDFSQTTIS RFEALNLSFK NMCKLKPLLE KWLNDAENLS SDSTASSPSA L
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.