Occludin/ELL Domain Containing Protein 1 (OCEL1) Belongs to the ELL/occludin family.ELL2 (RNA polymerase II elongation factor ELL2) is a 640aa nuclear protein that belongs to the ELL/occludin family.
This family is defined by a highly conserved domain of approximately 100 amino residues found within all eukaryotic occludin proteins and the RNA polymerase II elongation factor ELL.
These elongation factors activate elongation by suppressing transient pausing by polymerase at many sites along the DNA and govern its interaction with RNA polymerase II and the ternary elongation complex.
ELL2 may also contain a novel type of RNA polymerase II interaction domain that is capable of negatively regulating polymerase activity in promoter-specific transcription initiation in vitro.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human OCEL1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met1-Ala109 of human OCEL1, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
