Osteocalcin (OC) is the most abundant noncollagenous protein in mature bone where it constitutes 1% to 2% of the total protein.
Synthesized by osteoblasts, it is incorporated into the bone matrix
Osteocalcin is a 49-residue protein with three gamma-carboxyglutamic acid residues, at positions 17, 21 and 24.
These three residues confer on it a very strong ability to bind to hydroxyapatite.
Osteocalcin is a noncollagenous protein found in bone and dentin.
It is secreted by osteoblasts and thought to play a role in mineralization and calcium ion homeostasis.
It has been stipulated that osteocalcin may also function as a negative regulator of bone formation, although its exact role is unknown
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：chicken
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes chicken OC.
Isotype：IgY
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Ch
Immunogen：Recombinant protein corresponding to Ala21-Val97 of chicken OC, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
