Bidirectional transport of macromolecules between the cytoplasm and nucleus occurs through nuclear pore complexes (NPCs) embedded in the nuclear envelope.
NPCs are composed of subcomplexes, and NUP85 is part of one such subcomplex, Nup107-160
By proteomic analysis and mass spectrometry, Cronshaw et al.
(2002) identified 94 proteins associated with NPCs purified from rat liver nuclei.
Database analysis identified FLJ12549, which Cronshaw et al.
(2002) called NUP75, as the human homolog of a rat protein found in relative abundance, with 16 copies per NPC.
The deduced human protein has a calculated molecular mass of 75kD and is homologous to the yeast Nup85 protein.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse NUP85.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Gln418-Leu653 of mouse NUP85, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
