84-4430-69　NUP85 (Nucleoporin 85kD, Nup75, FROUNT, Nuclear pore complex protein Nup85, Pericentrin-1) 200ul　364460

特徴

• Bidirectional transport of macromolecules between the cytoplasm and nucleus occurs through nuclear pore complexes (NPCs) embedded in the nuclear envelope.
• NPCs are composed of subcomplexes, and NUP85 is part of one such subcomplex, Nup107-160
• By proteomic analysis and mass spectrometry, Cronshaw et al.
• (2002) identified 94 proteins associated with NPCs purified from rat liver nuclei.
• Database analysis identified FLJ12549, which Cronshaw et al.
• (2002) called NUP75, as the human homolog of a rat protein found in relative abundance, with 16 copies per NPC.
• The deduced human protein has a calculated molecular mass of 75kD and is homologous to the yeast Nup85 protein.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
• AA Sequence:MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- R QFSSQVSILS AMELIWNLCE ILFIEVAPAG PLLLHLLDWV RLHVCEVDSL SADVLGSENP SKHDSFWNLV TILVLQGRLD EARQMLSKEA DASPASAGIC RIMGDLMRTM PILSPGNTQT LTELELKWQH WHEECERYLQ DSTFATSPHL ESLLKIMLGD EAALLEQKEL LSNWYHFLVT RLLYSNPTVK PIDLHYYAQS SLDLFLGGES SPEPLDNILL AAFEFDIHQV IKECSIAL
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.