Nucleoporin 50kD is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import.
Pseudogenes of this gene are found on chromosomes 5, 6, and 14.
NUP50 is a tristable switch that alternates between binding modes
The C terminus binds importin-beta through RAN-GTP.
The N terminus binds the C terminus of importin-alpha, while a central domain binds importin-beta.
NUP50:importin-alpha:importin-beta binds cargo and can stimulate nuclear import.
Endogenous NUP50 can shuttle and is accessible from the cytoplasmic side of the nuclear envelope.
These findings identified NUP50 as a cofactor for importin-alpha:importin-beta nuclear import and as a subunit of the importin complex.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat NUP50.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Pro179-Val428 foil or amber vial rat NUP50, fused to two N-terminal Tags, His-Tag and GST-tag, expressed in E. coli.
