84-4430-65　NUP50 (Nucleoporin 50kD, NPAP60, NPAP60L, Nuclear pore-associated protein 60kD-like) 200ul　364456

特徴

• Nucleoporin 50kD is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import.
• Pseudogenes of this gene are found on chromosomes 5, 6, and 14.
• NUP50 is a tristable switch that alternates between binding modes
• The C terminus binds importin-beta through RAN-GTP.
• The N terminus binds the C terminus of importin-alpha, while a central domain binds importin-beta.
• NUP50:importin-alpha:importin-beta binds cargo and can stimulate nuclear import.
• Endogenous NUP50 can shuttle and is accessible from the cytoplasmic side of the nuclear envelope.
• These findings identified NUP50 as a cofactor for importin-alpha:importin-beta nuclear import and as a subunit of the importin complex.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
• AA Sequence:MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- NNSNQPP SSGPASSTAC PGNAYHKQLA GLNCSVRDWI VKHVNTNPLC DLTPIFKDYE RYLATIEKQL ENGGGSSSES QTDRATAGME PPSLFGSTKL QQESPFSFHG NKAEDTSEKV EFTAEKKSDA AQGATSASFS FGKKIESSAL GSLSSGSLTG FSFSAGSSSL FGKDAAQSKA ASSLFSAKAS ESPAGGGSSE CRDGEEEEND EPPKVVVTEV KEEDAFYSKK CKLFYKKDNE FKEKGVGTLH LKPTATQKTQ LLV
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.