84-4430-62　NUP35 (Nucleoporin 35kD, MP44, NP44, Mitotic phosphoprotein 44, Nuclear pore complex protein Nup53) 200ul　364453

特徴

• Nucleoporin NUP53 is a member of the nucleoporin family.
• The protein is localized to the nuclear rim and is part of the nuclear pore complex (NPC).
• All molecules entering or leaving the nucleus either diffuse through or are actively transported by the NPC.MP44 has a calculated molecular mass of about 35kD.
• It has a small number of scattered phenylalanine-glycine repeats, but not the large domains seen in other nucleoporins.
• The central 150aa of MP44 share 21% identity with S.
• cerevisiae Nup53.
• Fluorescence-tagged MP44, transfected into HeLa cells, showed punctate nuclear rim localization typical of nucleoporins.
• Based on this localization and the homology of MP44 to other nucleoporins, Cronshaw et al.
• (2002) proposed that MP44 be renamed NUP35.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
• AA Sequence:MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- TGQSMFSPA SIGQPRKTTL SPAQLDPFYT QGDSLTSEDH LDDSWVTVFG FPQASASYIL LQFAQYGNIL KHVMSNTGNW MHIRYQSKLQ ARKALSKDGR IFGESIMIGV KPCIDKSVME SSDRCALSSP SLAFTPPIKT LGTPTQPGST PRISTMRPLA TAYKASTSDY QVISDRQTPK
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.