The NUMA protein was one of the first to be described as a cell cycle-related protein based on a distinct immunofluorescent staining pattern: in interphase, NUMA is present throughout the nucleus, and in mitosis, it localizes to the spindle apparatus (Lydersen and Pettijohn, 1980).
Some patients with autoimmune disease have antibodies directed against the NUMA protein.
The full-length NUMA cDNA (Compton et al., 1992; Yang et al., 1992) predicts a protein with a calculated molecular mass of about 237kD, containing 2 globular domains separated by a coiled-coil domain of 1,485aa.
Wong et al.
(2006) stated that the coiled-coil region of NUMA can form parallel coiled-coil dimers.
By fluorescence in situ hybridization, Sparks et al.
(1993) demonstrated that the NUMA1 gene is present in single copy and located on chromosome 11q13.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human NUMA1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Phe1700-His2115 of human NUMA1, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
