They assembled a full-length clone encoding a deduced 420aa protein, which they called NEFA, with a calculated molecular mass of 48kD.
The protein contains a basic amino acid-rich putative DNA-binding domain with a potential nuclear targeting signal, 2 helix-loop-helix motif regions, 2 concurrent EF-hand motifs, an acidic amino acid region between the EF-hands, and a leucine zipper motif.
The protein shares high homology with human NUCB1.
Isoelectric focusing demonstrated the presence of NUCB2 on the plasma membrane, in the cytosol, and in the culture supernatant.
NUCB2 is a highly charged protein with a relative molecular mass of 55kD by SDS-PAGE, despite the lack of N- or O-glycosylation sites.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse NUCB2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Val25-Thr420 of mouse NUCB2, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
