84-4430-52　NUAK1 (NUAK Family SNF1 Like Kinase 1, ARK5, OMPHK1, NuaK1, AMP-Activated Protein Kinase Family Member 5, Omphalocele kinase 1) 200ul　364443

特徴

• The deduced 661aa protein shares about 52% identity over 252aa with plant SNF1-related protein kinase.
• NUAK1 had an apparent molecular mass of about 75kD by SDS-PAGE.
• RT-PCR detected highest expression in heart and brain, followed by skeletal muscle, kidney, ovary, placenta, lung, and liver.
• Little to no expression was detected in other tissues examined.
• The phosphorylation was stimulated by AMP and did not require accessory binding proteins.
• Transfection of ARK5 also increased the survival of HepG2 cells exposed to glucose starvation and reduced oxygen tension.
• Increased cell survival was accompanied by phosphorylation of ARK5 on ser600 by AKT, which activated ARK5 kinase activity.
• Activated ARK5 phosphorylated ATM, which led to phosphorylation of p53.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.