Netrin-4 is a novel Netrin more closely related to laminin Beta chain that is broadly expressed in both neural and non-neural tissues.
It acts during the development and controls the migration of neurons.
The role of Netrin-4 in guiding neurones seems to be mediated by known Netrin-1 receptors: DCC and UNC5H1.
Netrin-4 is transcribed in a variety of cells including epithelial cells and vascular endothelial cells.
Recent studies suggest a role of Netrin-1 and 4 in the branching morphogenesis in the lung.
Netrin-4 promotes angiogenesis by stimulating proliferation and migration of microvascular endothelial cells to the same extent that vascular endothelial growth factor (VEGF).
Kidney also express Netrin-4 whose level is down regulated upon ischemia-reperfusion injury.
Netrin-4 has been reported to be a novel prognostic marker in breast cancer.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human Ntn4.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Glu349-His592 of human Ntn4, fused to two N-terminal Tags, His-Tag and GST-tag, expressed in E. coli.
