Neuropathy Target Esterase deacetylates intracellular phosphatidylcholine to produce glycerophosphocholine.
It is thought to function in neurite outgrowth and process elongation during neuronal differentiation.
The protein is anchored to the cytoplasmic face of the endoplasmic reticulum in both neurons and non-neuronal cells.
Mutations in this gene result in autosomal recessive spastic paraplegia, and the protein is the target for neurodegeneration induced by organophosphorus compounds and chemical warfare agents.
Multiple transcript variants encoding different isoforms have been found for this gene.
The deduced 1,327aa protein contains 4 predicted transmembrane domains and multiple potential sites for N- and O-linked glycosylation.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat NTE.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Leu965-Thr1137 of rat NTE, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
