Neurotrophin-4 (NT-4) like BDNF, is a neurotrophic factor that signals predominantly through the TrkB receptor tyrosine kinase.
It is also known as NT4, NT5, NTF4, and NT-4/5.
Differentiation, survival, and function of neurons in vertebrates are controlled by multiple, target-derived neurotrophic factors.
The best characterized mammalian neurotrophic factors are 4 structurally related 13- to 14kD basic proteins known as neurotrophinsBerkemeier et al.
(1992) identified a cluster of genes on human chromosome 19 that includes neurotrophin-5 (NT5) and 3 pseudogenes, designated NTF6A, NTF6B, and NTF6G.
The NTF5 and NTF6 gene loci were mapped to chromosome 19 by Southern analysis of somatic cell hybrid panels.
In the mouse, the Ntf5 gene was assigned to chromosome 7.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human NT4.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Gly81-Ala210 of human NT4, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
