ANP is a hormone important in maintaining body fluid and sodium homeostasis.
In cardiomyocytes, human ANP is synthesized as a 151aa prepropeptide.
After the signal peptide is removed by the signal peptidase, pro-ANP is stored in the dense granules of the cell.
On stimulation, pro-ANP is released from the dense granules and activated on the cell surface by proteolytic cleavage at residue arginine 98, generating an NH2-terminal propeptide and a 26aa COOH-terminal peptide that is biologically active.
Plasma concentration of N-terminal atrial natriuretic peptide (Nt-proANP) is strongly directly related to salt sensitivity.In contrast to Nt-proANP, Nt-proBNP is not related to salt sensitivity.
Salt sensitivity, as estimated by Nt-proANP, seems to be reduced in type 2 diabetes.
Both N-ANP and N-BNP identifies a greater number of patients at risk of death or heart failure than either peptide alone.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human NT-ProANP.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Ala25-Arg123 of human NT-ProANP, fused to two N-terminal Tags, His-Tag and GST-tag, expressed in E. coli.
