The neuregulins, including NRG4, activate type-1 growth factor receptors (EGFR) to initiating cell-to-cell signaling through tyrosine phosphorylation.Loss of expression of NRG4 is frequently seen in advanced bladder cancer while increased NRG4 expression correlates to better survival.
By searching databases for sequences similar to NRG1, Harari et al.
(1999) identified an Nrg4 EST clone originating from a mouse liver cDNA library
The deduced 115aa protein has a putative N-terminal EGF-like extracellular domain with 2 N-glycosylation sites, followed by a transmembrane region, and a short C-terminal cytoplasmic tail with another N-glycosylation site.
It also has 6 cysteines predicted to form 3 disulfide bridges within the extracellular domain.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse NRG4.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Met1-Ser62 of mouse NRG4, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
