Rev-ErbA beta is a member of the Rev-ErbA family of transcription factors.
Rev-erbBeta, like Rev-erbalpha, belongs to the nuclear receptor superfamily and can modulate gene expression by directly binding to their promoters.Rev-erbBeta is similar to Rev-erbalpha in its protein structure and function as a transcriptional repressor.
The crystal structure of an unliganded Rev-erbBeta ligand-binding domain (LBD) has been resolved (see figure to the right) and shows an extremely small ligand-binding pocket.
However, Rev-erbBeta has been shown to interact with heme, which appears important for its function.
The structure of Rev-erbBeta complexed with heme (see figure to the left) shows a substantial movement of helices 1 (N-terminus ) and 11 (C-terminus ) which opens up a large binding pocket in the interior of the protein that is able to accommodate this ligand.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat NR1D2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Lys249-Pro578 of rat NR1D2, fused to two N-terminal Tags, His-Tag and GST-tag, expressed in E. coli.
