NOX5 is distantly related to the gp91-phox subunit of the phagocyte NADPH oxidase with conserved regions crucial for electron transport (NADPH, FAD, and heme binding sites).
However, NOX5 has a unique N-terminal extension that contains 3 EF-hand motifs
Banfi et al.
(2001) demonstrated 2 NOX5 isoforms of 737 and 719aa designated NOX5A and NOX5B, respectively.
Northern blot analysis demonstrated restricted expression of an approximately 2.7-kb NOX5A transcript in spleen and an approximately 2.9-kb NOX5B transcript in testis.
In situ hybridization localized NOX5 in pachytene spermatocytes of testis and in B- and T-lymphocytic-rich areas of spleen and lymph nodes.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human NOX5.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Ser456-Phe765 of human NOX5, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
