84-4429-98　NOS3 (Nitric Oxide Synthase 3, Endothelial, ECNOS, NOS III, E-NOS, C-NOS, ENOS, CNOS, Constitutive NOS) 200ul　364390

特徴

• Nitric oxide (NO) is produced by a group of enzymes called nitric oxide synthases (NOS).
• These enzymes catalyze the production of NO and L-citrulline from L-arginine, O2, and NADPH-derived electrons.
• The cell types that express eNOS include vascular endothelial cells, cardiomyocytes and others.
• In blood vessels, NO produced by the eNOS in endothelial cells functions as a vasodilator thereby regulating blood flow and pressure.
• Mutant eNOS knockout mice have blood pressure that is 30% higher than wild-type littermates.
• Within cardiomyocytes, eNOS affects Ca2 currents and contractility.
• Expression of eNOS is usually reported to be constitutive, though modest degrees of regulation occur in response to factors such as shear stress, exercise, chronic hypoxia, and heart failure.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
• AA Sequence:MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- ATILYGSET GRAQSYAQQL GRLFRKAFDP RVLCMDEYDV VSLEHETLVL VVTSTFGNGD PPENGESFAA ALMEMSGSYN SSPRPEQHKS YKIRFNSVSC SDPLVSSWRR KRKESRNTDS AGALGTLRFC VFGLGSRAYP HFCAFARAVD TRLEELGGER LLQLGQGDEL CGQEEAFGGW AQAAF
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.