84-4429-87　NNE (Enolase, Non Neuronal, ENO1, ENO1L1, MPB1, PPH, Alpha Enolase, Enolase 1, Phosphopyruvate hydratase, Plasminogen-binding protein, 2-phospho-D-glycerate hydro-lyase, C-myc promoter-binding) 200ul　364379

特徴

• Enolase 1, more commonly known as alpha-enolase, is a glycolytic enzyme expressed in most tissues, one of the isozymes of enolase.
• It is a homodimer composed of 2 alpha subunits.
• Its gene, the ENO1, also encodes the Myc-binding protein-1(MBP1), which downregulates the activity of c-myc protooncogene.
• Alpha-enolase is the longer form (48kD) localized in both cytoplasm and nuclei, while MBP1 is shorter (37kD) and is found mostly in the nuclei.
• Alpha-enolase has been identified as an autoantigen in Hashimotos encephalopathy.
• Single studies have also identified it as an autoantigen associated with severe asthma and a putative target antigen of anti-endothelial cell antibody in Behets disease.
• Reduced expression of the enzyme has been found in the corneas of people suffering from keratoconus.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
• AA Sequence:MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MSILKIHARE IFDSRGNPTV EVDLFTSKGL FRAAVPSGAS TGIYEALELR DNDKTRYMGK GVSKAVEHIN KTIAPALVSK KLNVTEQEKI DKLMIEMDGT ENKSKFGANA ILGVSLAVCK AGAVEKGVPL YRHIADLAGN SEVILPVPAF NVINGGSHAG NKLAMQEFMI LPVGAANFRE AMRIGAEVYH NLKNVIKEKY GKDATNVGDE GGFAPNILEN KEGLELLKTA IGKAGYTDKV VIGMDVAASE FFRSGKYDLD FKSPDDPSRY ISPDQLADLY KSFIKDYPVV SIEDPFDQDD WGAWQKFTAS AGIQVVGDDL TVTNPKRIAK AVNEKSCNCL LLKVNQIGSV TESLQACKLA QANGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL LRIEEELGSK AKFAGRNFRN PLAK
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.