Neuromedin U is a neuropeptide found in the brain of humans and other mammals, which has a number of diverse functions including contraction of smooth muscle, regulation of blood pressure, pain perception, appetite, bone growth, and hormone release.
Neuromedin U exists as multiple isoforms (eg.
25 and 8aa in humans) in some mammals, but only as a single isoform (eg.
23aa in rats) in others.
The relative contribution of the different isoforms to the biological function of neuromedin U is generally not well understood.
Neuromedin U, like many neuroactive peptides, is amidated at the C-terminus.
The sequence of neuromedin U-23 in rats is: YKVNEYNGPVAPSGGFFLFRPRN-(NH2)
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.