NMES1 shares 31 to 34%aa identity with the mouse, bovine, and human NADH-ubiquinone oxidoreductase MLRQ subunit.
Northern blot analysis detected a transcript of 0.9 kb in esophagus, stomach, small intestine, colon, and placenta.
Very weak expression was detected in lung, and no expression was detected in several other tissues.
Immunohistochemical staining revealed expression localized to nuclei of the basal cell layer of normal esophagus.
In situ hybridization of embryonic mice revealed expression in the ossification center and spinal cord 8 days postcoitum.
Expression extended throughout bone tissue and vertebrae from this stage until birth.
Other sites of abundant expression included the developing brain, intestine, and stomach.
Weak expression was detected in the developing mouse lung.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-2ug/mlImmunocytochemistry: 5-20ug/mlImmunohistochemistry (Paraffin): 5-20ug/mlOptimal dilutions to be determined by the researcher.