The nucleoside diphosphate (NDP) kinases are ubiquitous enzymes that catalyze transfer of gamma-phosphates, via a phosphohistidine intermediate, between nucleoside and dioxynucleoside tri- and diphosphates.
The enzymes are products of the NM23 gene family.
Munier et al.
(1998) identified a novel homolog of NM23 in the EST database.
They used PCR to clone the full coding sequence from several human cDNA libraries.
The NME5 gene encodes a 212aa polypeptide with homology to the NDP kinases.
Northern blot analysis detected abundant expression of a 1.1-kb mRNA in testis.
In situ hybridization to testis revealed NME5 mRNA in the seminiferous tubules in a pattern consistent with stage-specific expression in germinal cells during spermatogenesis.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human NME5.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Ile3-Lys194 of human NME5, fused to His-Tag and GST-tag at N-terminal expressed in E. coli.
