Neuromedin B (NMB) is a bombesin-related peptide in mammals.
It was originally purified from pig spinal cord, and later shown to be present in human central nervous system and gastrointestinal tract.
NMB acts by binding to its high affinity cell surface receptor, neuromedin B receptor (NMBR).
This receptor is a G protein-coupled receptor with seven transmembrane spanning regions, hence the receptor is also denoted as a 7-transmembrane receptor (7-TMR).
Upon binding several intracellular signaling pathways are triggered.
When NMB binds to its 7-TMR, the heterotrimeric G protein that is attached to the receptor is activated.
The G-protein is called heterotrimeric because it consists of 3 polypeptides: alpha subunit, beta subunit, and gamma subunit.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human NMB.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Ala25-Lys121 of human NMB, fused to His-Tag and GST-tag at N-terminal expressed in E. coli.
