NKRF encodes a transcription factor that interacts with specific negative regulatory elements (NREs) to mediate transcriptional repression of certain NK-kappa-B-responsive genes.
The protein localizes predominantly to the nucleolus with a small fraction found in the nucleoplasm and cytoplasm.
The deduced 388aa protein has a calculated molecular mass of 43.8 kD and contains an N-terminal nuclear localization signal.
The C-terminal 92aa of NRF constitute the DNA-binding domain, which has 4 turns and 4 alpha helices, similar to a winged helix-turn-helix DNA-binding motif.
Northern blot analysis detected ubiquitous expression of 3.7- and 4.0-kb transcripts that differ in their 3-prime untranslated regions.
The longer transcript predominated in most tissues, but the 3.7-kb transcript predominated in brain.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human NKRF.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met1-Val296 of human NKRF, fused to His-Tag and GST-tag at N-terminal expressed in E. coli.
