2B4, called also NKR2B4 [natural killer cell receptor 2B4] has been identified originally on mouse NK-cells and subsets of T-cells mediating nonMHC restricted cell killing.
Murine NK-cells express two alternatively spliced isoforms, designated 2B4-S and 2B4-L.
2B4-S functions as an activating receptor, whereas 2B4-L is inhibitory.
The new designation of this protein is CD244
The 2B4 protein is expressed on all NK-cells, a subset of CD8(+) T-cells, basophils, mast cells, and monocytes.
2B4 is considered to be an activating receptor because cross-linking of 2B4 on NK-cells with a monoclonal antibody leads to NK-cell activation in vitro.
However, 2B4 can also act as an inhibitory signaling receptor for NK-cells.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse NKR2B4.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Ser26-Thr212 of mouse NKR2B4, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
