The Na-K-Cl cotransporters are a family of integral membrane proteins that mediate the coupled transport of Na+, K+, and Cl- across the plasma membrane.NKCC2 protein is encoded in 26 exons, and the coding region spans 80 kb of genomic DNA with introns that range in length from 120 bp to 15 kb.
The location of intron/exon boundaries are very similar to those seen in SLC10A3 through exon 19.
Three alternative forms of exon 4 are encoded in genomic DNA, as reported in other species.
The gene, symbolized SLC12A1 in human and mouse, is structurally related to another Na-K-Cl cotransporter (SLC12A2; previously called NKCC1) which, unlike the kidney-specific SLC12A1, is expressed in many tissues, including the basolateral membrane of secretory epithelia, where it mediates active chloride secretion.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human NKCC2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Arg814-Ser1099 of human NKCC2, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
