The sodium-iodide symporter (NIS) is an ion pump that actively transports iodide (I-) across the basolateral membrane into thyroid epithelial cells.
The NIS a member of the sodium/solute symporter family.
Active transport of iodine into the thyroid gland is an important step in the process of iodide organification and the formation of triiodothyronine (T3) and thyroxine (T4).
The symporter is stimulated by TSH, thereby increasing iodine uptake and iodination of tyrosine.
The pump symports two sodium ions for every iodide ion.
This symporter is driven by low internal sodium concentration in the thyroid epithelial cells, via facilitated diffusion, caused by the sodium/potassium ATPase pump.
This pump is the target of certain anion based inhibitors such as perchlorate, pertechnetate, and thiocyanate.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human NIS.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Ser547-Leu643 of human NIS, fused to two N-terminal Tags, His-Tag and GST-tag, expressed in E. coli.
