Ninein is one of the proteins important for centrosomal function.
This protein is important for positioning and anchoring the microtubules minus-ends in epithelial cells.
Localization of this protein to the centrosome requires three leucine zippers in the central coiled-coil domain.
Multiple alternatively spliced transcript variants that encode different isoforms have been reported.The deduced 2,047aa protein has a calculated molecular mass of 239kD.
NIN contains an N-terminal GTP-binding site, a large coiled-coil domain with 4 leucine zipper motifs, and a C-terminal domain responsible for GSK binding.
Unlike mouse Nin, it does not have an N-terminal Ca(2+)-binding site.
Northern blot analysis detected a 9.0-kb transcript in all tissues examined and a 7.0-kb transcript predominantly in heart and skeletal muscle.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human NIN.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met1-Glu153 of human NIN, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
