Entactin (or nidogen) is a component of the basement membrane alongside other components such as collagen type IV, proteoglycans ( heparan sulfate and glycosaminoglycans), laminin and fibronectin.
Entactin (or nidogen) is a member of the nidogen family of basement membrane glycoproteins.
The protein interacts with several other components of basement membranes, and may play a role in cell interactions with the extracellular matrix
Entactin, a 150kD sulfated glycoprotein, is a major component of basement membranes and forms a highly stable noncovalent complex with laminin.
Durkin et al.
(1988) derived the complete amino acid sequence of mouse entactin from sequencing of cDNA clones.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human NID.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Ala971-Cys1219 of human NID, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
